John D. Griffin, Ph.D.
My lab's research efforts are focused on determining the cellular machanisms involved in thermoregulatory control. More specifically, we are interested in how set-point temperature is elevated into the hyperthermic range during an immune response (fever) or acute surge in hormone release (hot flash).
Current Funding:
NIH AREA Grant: The Responses of Thermoregulatory Neurons to CGRP
Period: 1/23/06-123/09
Total Direct Costs: $150,000
Current Projects & Student:
Determine the responses of anterior hypothalamic (AH) thermoregulatory neurons to the alpha-1 adrenoreceptor agonist, Cirazoline.
Method: Single-unit recording from AH neurons in an in-vitro tissue slice preparation.
Students: Mitra Irdmusa, Mark Streer, Andrew Speidell, Ted Imbrey
Collaborator: Clark Blatteis (University of Tennessee, Memphis)
Determine the thermosensitivity and neurotransmitter phenotype of AH neurons which project to the dorsomedial hypothalamus.
Method: Whole-cell recording & in-vitro retrograde labeling of AH neurons in tissue slices.
Students: Emily Sherbin, Connie Chung
Collaborator: Christopher Del Negro (Applied Science, College of William and Mary)
Method: Histochemical staining to identify the presence of the mGlutamate transporter.
Students: Kathy Bowman, Megan Luteran, Swati Mishra
Current Funding:
NIH AREA Grant: The Responses of Thermoregulatory Neurons to CGRP
Period: 1/23/06-123/09
Total Direct Costs: $150,000
Current Projects & Student:
Determine the responses of anterior hypothalamic (AH) thermoregulatory neurons to the alpha-1 adrenoreceptor agonist, Cirazoline.
Method: Single-unit recording from AH neurons in an in-vitro tissue slice preparation.
Students: Mitra Irdmusa, Mark Streer, Andrew Speidell, Ted Imbrey
Collaborator: Clark Blatteis (University of Tennessee, Memphis)
Determine the thermosensitivity and neurotransmitter phenotype of AH neurons which project to the dorsomedial hypothalamus.
Method: Whole-cell recording & in-vitro retrograde labeling of AH neurons in tissue slices.
Students: Emily Sherbin, Connie Chung
Collaborator: Christopher Del Negro (Applied Science, College of William and Mary)
Method: Histochemical staining to identify the presence of the mGlutamate transporter.
Students: Kathy Bowman, Megan Luteran, Swati Mishra
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